In its statement announcing the air defence zone on Saturday, the Chinese defence ministry said aircraft must report a flight plan, "maintain two-way radio communications", and "respond in a timely and accurate manner" to identification inquiries.
"China's armed forces will adopt defensive emergency measures to respond to aircraft that do not co-operate in the identification or refuse to follow the instructions," the statement said.
Japan Airlines and All Nippon Airlines said on Tuesday they would stop filing flight plans demanded by China on routes through the zone following a request from the Japanese government.
Singapore Airlines and Australia's Qantas have both said they will abide by the new rules.
The US has flown two B-52 bombers over disputed islands in the East China Sea in defiance of new Chinese air defence rules, officials say.
US Colonel Steve Warren at the Pentagon said Washington had "conducted operations in the area of the Senkakus".
"We have continued to follow our normal procedures, which include not filing flight plans, not radioing ahead and not registering our frequencies," he said.
There had been no response from China, he added.
The aircraft, which were unarmed, had taken off from Guam on Monday and the flight was part of a regular exercise in the area, US defence officials said. Both planes later returned to Guam.
"China's armed forces will adopt defensive emergency measures to respond to aircraft that do not co-operate in the identification or refuse to follow the instructions," the statement said.
Japan Airlines and All Nippon Airlines said on Tuesday they would stop filing flight plans demanded by China on routes through the zone following a request from the Japanese government.
Singapore Airlines and Australia's Qantas have both said they will abide by the new rules.
The US has flown two B-52 bombers over disputed islands in the East China Sea in defiance of new Chinese air defence rules, officials say.
US Colonel Steve Warren at the Pentagon said Washington had "conducted operations in the area of the Senkakus".
"We have continued to follow our normal procedures, which include not filing flight plans, not radioing ahead and not registering our frequencies," he said.
There had been no response from China, he added.
The aircraft, which were unarmed, had taken off from Guam on Monday and the flight was part of a regular exercise in the area, US defence officials said. Both planes later returned to Guam.
