Study of CAPE Effect on Apoptosis Induction in AGS Human Gastric Cancer Cell Line

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Article Information​

Jundishapur Journal of Natural Pharmaceutical Products: Vol.11, issue 1; e22534
Published Online: January 12, 2016
Article Type: Research Article
Received: August 5, 2014
Revised: October 18, 2014
Accepted: October 29, 2014
DOI: https://doi.org/10.17795/jjnpp-22534
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Study of CAPE Effect on Apoptosis Induction in AGS Human Gastric Cancer Cell Line​

Authors:

Nematallah Amini-Sarteshnizi1 ,

Hossein Teimori2 , * ,

Pezhman Beshkar3 ,

Roghayeh Amini-Sarteshnizi4 ,

Morteza Nikoukar5
1Department of Biology, Faculty of Sciences, University of Mohaghegh Ardabili, Ardabil, IR Iran
2Cellular and Molecular Research Center, Shahrekord University of Medical Sciences, Shahrekord, IR Iran
3Laboratory Hematology and Blood Bank, High Institute for Blood Transfusion Education, Tehran, IR Iran
4Department of Food Science and Technology, Faculty of Food Science and Technology, Shahid Beheshti University of Medical Sciences, Tehran, IR Iran
5Medical Plants Research Center, Shahrekord University of Medical Sciences, Shahrekord, IR Iran
* Corresponding author: Hossein Teimori, Cellular and Molecular Research Center, Shahrekord University of Medical Sciences, Shahrekord, IR Iran. Tel: +98-3813346692; +98-9133054781, Fax: +98-3813330709, E-mail: [email protected]

 

[ginfreely]

Article Information​

Jundishapur Journal of Natural Pharmaceutical Products: Vol.11, issue 1; e22534
Published Online: January 12, 2016
Article Type: Research Article
Received: August 5, 2014
Revised: October 18, 2014
Accepted: October 29, 2014
DOI: https://doi.org/10.17795/jjnpp-22534
CrossmarkChecking
Navigate to

Study of CAPE Effect on Apoptosis Induction in AGS Human Gastric Cancer Cell Line​

Authors:

Nematallah Amini-Sarteshnizi1 ,

Hossein Teimori2 , * ,

Pezhman Beshkar3 ,

Roghayeh Amini-Sarteshnizi4 ,

Morteza Nikoukar5
1Department of Biology, Faculty of Sciences, University of Mohaghegh Ardabili, Ardabil, IR Iran
2Cellular and Molecular Research Center, Shahrekord University of Medical Sciences, Shahrekord, IR Iran
3Laboratory Hematology and Blood Bank, High Institute for Blood Transfusion Education, Tehran, IR Iran
4Department of Food Science and Technology, Faculty of Food Science and Technology, Shahid Beheshti University of Medical Sciences, Tehran, IR Iran
5Medical Plants Research Center, Shahrekord University of Medical Sciences, Shahrekord, IR Iran
* Corresponding author: Hossein Teimori, Cellular and Molecular Research Center, Shahrekord University of Medical Sciences, Shahrekord, IR Iran. Tel: +98-3813346692; +98-9133054781, Fax: +98-3813330709, E-mail: [email protected]

Wow Iran got alot of papers on stomach cancer

 

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Wow Iran got alot of papers on stomach cancer

Of course it’s because stomach cancer is one of top cancers there. Only China with top stomach cancer but disproportionate contribution

 

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Wow Iran got alot of papers on stomach cancer

Taiwan also got many papers on stomach cancer.

 

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Abstract​

Background:​

Propolis is a natural product of bee and caffeic acid phenethyl ester (CAPE) is a pharmacologically important product of propolis.

Objectives:​

The aim of this study was to investigate the effect of CAPE on apoptosis induction in AGS human gastric cancer cells.

Materials and Methods:​

The cytotoxic effects of CAPE at different concentrations were investigated on AGS cells viability after 24 hours treatment by MTT assay. To measure the effect of CAPE on apoptosis induction, AGS cells were treated with CAPE for 24 hours and investigated by FITC Annexin V/PI staining using flow cytometry.

Results:​

CAPE prevented growth and proliferation of AGS human gastric cancer cell line in a concentration-dependent manner with an IC50 of approximately 60 µM by a 24-hour treatment. Also CAPE caused increased induction of apoptosis in AGS cells from 1.37 % in control cells to 21.76 % in treated cells with 30 µM CAPE.

Conclusions:​

CAPE prevents growth and proliferation of AGS human gastric cancer cell line through inducing programmed cell death in AGS cells. Therefore, CAPE could be helpful for developing chemotherapeutic agents or as an adjuvant for human gastric cancer treatment.